Multi-Parametric Live Cell Microscopy of 3D Tissue Models
| By: | Author |
| Publisher: | Springer Nature |
| Print ISBN: | 9783319673578 |
| eText ISBN: | 9783319673585 |
| Edition: | 0 |
| Copyright: | 2017 |
| Format: | Page Fidelity |
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This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.