Human Pluripotent Stem Cells: A Practical Guide
| By: | Uma Lakshmipathy; Chad C. MacArthur; Mahalakshmi Sridharan; Rene H. Quintanilla |
| Publisher: | Wiley Global Research (STMS) |
| Print ISBN: | 9781119394334 |
| eText ISBN: | 9781119394365 |
| Edition: | 1 |
| Copyright: | 2018 |
| Format: | Reflowable |
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Comprehensive coverage of the entire induced pluripotent stem cell basic work flow
Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.
Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.
- Includes helpful tips and tricks that are normally omitted from regular research papers
- Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations
- Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner
- Written by experts with extensive experience in stem cell technologies
Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.