Correlative Light and Electron MIcroscopy
| By: | Mueller-Reichert, Thomas |
| Publisher: | Elsevier S & T |
| Print ISBN: | 9780124160262 |
| eText ISBN: | 9780123914385 |
| Edition: | 0 |
| Format: | Reflowable |
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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.